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Histone H3 (di methyl K79) Rabbit polyclonal antibody

Histone H3 (di methyl K79) Rabbit polyclonal antibody

货号: S0B0544
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    H3K79me2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB

  • 反应种属 ?

    Hu, Ms

  • 预测反应种属
    (反应种属缩写表)

    Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe

  • 纯化方式

    Protein A

  • 浓度

    0.8 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
Dot Blot 1:1000
WB 1:1000
ICC 1:500
ICFCM 1:800
背景介绍

  • H3K79me2 is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the di-methylation at the 79th lysine residue of the histone H3 protein. H3K79me2 is detected in the transcribed regions of active genes.

  • 免疫印迹

    • WB result of Histone H3 (di methyl K79) Rabbit pAb
      Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa
      Exposure time: 3 s (This blot was developed with high sensitivity substrate)

    • WB result of Histone H3 (di methyl K79) Rabbit pAb
      Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa
      Exposure time: 3 s (This blot was developed with high sensitivity substrate)

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Histone H3 (di methyl K79) antibody at 1/800 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Histone H3 (di methyl K79) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 配对推荐

    • Dot blot result of Histone H3 (di methyl K79) Rabbit pAb
      Lane 1: H3 (di methyl K79) peptide
      Lane 2: H3 (mono methyl K79) peptide
      Lane 3: H3 (tri methyl K79) peptide
      Lane 4: H3 unmodified peptide
      Primary antibody: Histone H3 (di methyl K79) Rabbit pAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Exposure time: 90 s