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Histone H3 (mono methyl K36) Rabbit polyclonal antibody

Histone H3 (mono methyl K36) Rabbit polyclonal antibody

货号: S0B0525
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (mono methyl K36)

  • 分子别名

    H3K36me

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 应用

    ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:500
背景介绍

  • H3K36me is an epigenetic modification to the DNA packaging protein Histone H3, specifically, the mono-methylation at the 36th lysine residue of the histone H3 protein. The methylation of H3K36 has particularly had effects in transcriptional repression, alternative splicing, dosage compensation, DNA replication and repair, DNA methylation, and the transmission of the memory of gene expression from parents to offspring during development.

  • 免疫印迹

    • WB result of Histone H3 (mono methyl K36) Rabbit pAb
      Primary antibody: Histone H3 (mono methyl K36) pAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (mono methyl K36) Rabbit pAb
      Primary antibody: Histone H3 (mono methyl K36) pAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: Neuro-2a whole cell lysate 20 µg
      Lane 3: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (mono methyl K36) Rabbit pAb
      Primary antibody: Histone H3 (mono methyl K36) pAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg
      Lane 3: rat liver lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa

  • 斑点杂交

    • Dot blot result of Histone H3 (mono methyl K36) Rabbit pAb
      Lane 1: H3 (mono methyl K36) peptide
      Lane 2: H3 (di methyl K36) peptide
      Lane 3: H3 (tri methyl K36) peptide
      Lane 4: H3 unmodified peptide
      Primary antibody: Histone H3 (mono methyl K36) Rabbit pAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Histone H3 (mono methyl K36) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).