12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
an RNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28 binds to the let-7 pre-microRNA and blocks production of the mature let-7 microRNA in mouse embryonic stem cells. In pluripotent embryonal carcinoma cells, LIN28 is localized in the ribosomes, P-bodies and stress granules. LIN28 is highly expressed in human embryonic stem cells[14] and can enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. LIN28 is a marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of iPS cells from human fibroblasts.
WB result of LIN28 Rabbit mAb
Primary antibody: LIN28 Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: JAR whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 26 kDa
LIN28 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating LIN28 in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using LIN28 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: T-47D whole cell lysate 20 µg (Input)
Lane 2: LIN28 Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 23 kDa
Observed MW: 26 kDa
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