PBS, 0.1% BSA, 0.01% Proclin 300
应用 | 稀释度 |
---|---|
FCM | 5 μl per million cells in 100μl volume |
CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It is a type of white blood cell that helps fight infection by triggering your immune system to destroy viruses, bacteria, and other germs that may make you sick. CD4 is also a receptor for the HIV virus, and when the virus infects cells with CD4 surface proteins, it depletes the number of T cells, B cells, natural killer cells, and monocytes in the patient's blood.
Flow cytometric analysis of Human CD4 expression on human PBMC (Human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 488 Mouse Anti-Human CD3 antibody and either Alexa Fluor® 647 Rabbit IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Rabbit Anti-Human CD4 Antibody (Right panel) at 1 μg/test. Fluorescent histograms were gated events with lymphocytes. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.