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Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb (S-748-20)

Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb (S-748-20)

货号: S0B0518
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Glycogen synthase kinase-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P49841

  • 克隆号

    S-748-20

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Ms, Rt, Sq, Xe

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20°C as supplied

稀释度
应用 稀释度
Dot Blot 1:1000
WB 1:1000
IP 1:50
背景介绍

  • GSK-3 is a serine/threonine protein kinase that phosphorylate either threonine or serine, and this phosphorylation controls a variety of biological activities, such as glycogen metabolism, cell signaling, cellular transport, and others. GSK-3 has also been shown to regulate immune and migratory processes. GSK-3 participates in a number of signaling pathways in the innate immune response, including pro-inflammatory cytokine and interleukin production. GSK-3 is also integrally tied to pathways of cell proliferation and apoptosis. GSK-3 has been shown to phosphorylate Beta-catenin, thus targeting it for degradation. GSK-3 is therefore a part of the canonical Beta-catenin/Wnt pathway, which signals the cell to divide and proliferate. Insulin indirectly inactivates GSK3 via downstream phosphorylation of the specific serine residues Ser21 and Ser9 in GSK-3 isoforms α and β, respectively, via the PI3K/Akt pathway (protein kinase B).

  • 免疫印迹

    • WB result of Phospho-GSK-3β (Ser9) Rabbit mAb
      Primary antibody: Phospho-GSK-3β (Ser9) Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HeLa starve overnight , then treated with Calyculin A (100nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47 kDa
      Observed MW: 45 kDa
      Exposure time: 180 s

    • WB result of Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb
      Primary antibody: Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 starve for 3hr, then treated with Calyculin A (100nM/ml, 30min) whole cell lysate 20 µg
      Negative control: Untreated NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 47 kDa
      Observed MW: 50kDa

  • 免疫沉淀

    • Phospho-GSK-3β (Ser9) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-GSK-3β (Ser9) in 0.4 mg HeLa starve overnight, then treated with Calyculin A (100nM) for 30 minutes whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-GSK-3β (Ser9) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HeLa starve overnight, then treated with Calyculin A (100nM) for 30 minutes whole cell lysate 20 µg (Input)
      Lane 2: Phospho-GSK-3β (Ser9) Rabbit mAb IP in HeLa starve overnight, then treated with
      Calyculin A (100nM) for 30 minutes whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa starve overnight, then treated with Calyculin A (100nM) for 30 minutes whole cell lysate
      Predicted MW: 47 kDa
      Observed MW: 47 kDa
      Exposure time: 30 s（This blot was developed with high sensitivity substrate）

  • 斑点杂交

    • Dot blot result of Phospho-GSK-3β (Ser9) Rabbit mAb
      Lane 1: Phospho-GSK-3β (Ser9) peptide
      Lane 2: Phospho-GSK-3β peptide
      Primary antibody: Phospho-GSK-3β (Ser9) Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Exposure time: 18 s