12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| FCM | 1:2000 |
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen-presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Flow cytometric analysis of mouse primary splenocytes labeling CD8α at 1/2000 dilution (right panel) compared with a Rat IgG, monoclonal Isotype Control (left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Then cells were stained with CD4 - Alexa Fluor® 647 separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
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