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CaMKII-α Recombinant Rabbit mAb (S-R329)

CaMKII-α Recombinant Rabbit mAb (S-R329)

货号: S0B0496
价格: 260
规格: 10μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 分子别名

    Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968
  • 细胞定位

    Cell projection, Synapse
  • Accession

    Q9UQM7
  • 克隆号

    S-R329
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC-P 1:100
背景介绍
  • CaMKII-α is an enzyme that belongs to the serine/threonine-specific protein kinase family, as well as the Ca2+/calmodulin-dependent protein kinase II subfamily. Ca2+ signaling is crucial for several aspects of synaptic plasticity at glutamatergic synapses. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity.

  • 免疫印迹

    • WB result of CaMKII-α Rabbit mAb
      Primary antibody: CaMKII-α Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 50, 60 kDa

    • WB result of CaMKII-α Rabbit mAb
      Primary antibody: CaMKII-α Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 50, 60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CaMKII-α antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CaMKII-αantibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.