PBS, 0.1% BSA, 0.01% Proclin 300
| 应用 | 稀释度 |
|---|---|
| FCM | 5 μl per million cells in 100μl volume |
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. CD3 is associated with an heterodimer creating the T-cell receptor (TCR). The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines.
Flow cytometric analysis of Mouse CD3 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD19 antibody and either Alexa Fluor® 594 Rat IgG2b Isotype Control (Left panel) or SDT Alexa Fluor® 594 Rat Anti-Mouse CD3 Antibody (17A2) (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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