12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
| IP | 1:50 |
TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a low density lipoprotein receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This protein is up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. TMPRSS2 participates in proteolytic cascades necessary for normal physiological function of the prostate.
WB result of TMPRSS2 Rabbit mAb
Primary antibody: TMPRSS2 Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 22, 55 kDa
(This blot was developed with high sensitivity substrate)
TMPRSS2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating TMPRSS2 in 0.4 mg LnCap whole cell lysate.
Western blot was performed on the immunoprecipitate using TMPRSS2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: LnCap whole cell lysate 20 µg (Input)
Lane 2: TMPRSS2 Rabbit mAb IP in LnCap whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in LnCap whole cell lysate
Predicted MW: 54 kDa
Observed MW: 22, 55 kDa
This blot was developed with high sensitivity substrate
IHC shows positive staining in paraffin-embedded human colon. Anti-TMPRSS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TMPRSS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-TMPRSS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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