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MTAP Recombinant Rabbit mAb (S-R363)

MTAP Recombinant Rabbit mAb (S-R363)

货号: S0B0492
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MSAP

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q13126

  • 克隆号

    S-R363

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:100
ICFCM 1:50
背景介绍

  • S-methyl-5'-thioadenosine phosphorylase (MTAP) is an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. It is responsible for the first step in this pathway, where it catalyzes the reversible phosphorylation of MTA to adenine and 5-methylthioribose-1-phosphate. This takes place after MTA is generated from S-adenosylmethionine.

  • 免疫印迹

    • WB result of MTAP Rabbit mAb
      Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: HT-29 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Negative control: A549 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 31 kDa

    • WB result of MTAP Rabbit mAb
      Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: RAW 264.7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 31 kDa

    • WB result of MTAP Rabbit mAb
      Primary antibody: MTAP Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 31 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling MTAP antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-MTAP antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).