Alexa Fluor® 647 Antibody Labeling Kit

Ideal antibody concentration is 5 mg/ml can be used. Antibodies > 5 mg/ml or < 2 mg/ml should be diluted /concentrated.
The recommended buffer system for antibodies is only PBS, pH 7.2-7.4.

Flow cytometric analysis of mouse primary splenocytes labeling CD8α (Alexa Fluor® 647 Conjugate) antibody (S0B0125) at 1/200 (1 μg) dilution / (right panel) compared with a Rabbit IgG, Isotype Control / (left panel). Cells were surface stained with CD4-APC-Cy7, then stained with Rabbit IgG (Left) / CD8α (Right) separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.

ICC shows positive staining in HeLa cells. Anti-Vimentin (Alexa Fluor® 647 Conjugate) antibody (S0B0146) was used at 1/250 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody (S0B0119) was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody (S0B0119) was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human colon cancer. Anti-Vimentin (Alexa Fluor® 647 Conjugate) antibody (S0B0146) was used at 1/250 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.