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Alexa Fluor® 647 Mouse Anti-Human CD21 Antibody (S-764-18)

Alexa Fluor® 647 Mouse Anti-Human CD21 Antibody (S-764-18)

货号: S0B0178
价格: 650
规格: 20T
介绍: -
其他: -
产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    CD21

  • 分子别名

    Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor (EBV receptor)

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cell membrane

  • Accession

    P20023

  • 克隆号

    S-764-18

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 应用

    FCM

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein G

  • 浓度

    20μg/ml

  • 标记

    Alexa Fluor® 647

  • 性状

    Liquid

  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度
FCM 5 μl per million cells in 100μl volume
背景介绍

  • CD21 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d.B cells express CD21 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. CD21 interacts with CD19 and on mature B cells forms a complex with CD81 (TAPA-1). The CD21-CD19-CD81 complex is often called the B cell co-receptor complex, because CD21 binds to opsonized antigens through attached C3d (or iC3b or C3dg) when the B-cell receptor binds antigen. This results in the B cell having greatly enhanced response to the antigen. Epstein-Barr virus (EBV) can bind CD21, enabling EBV to enter and infect B cells.

  • 流式分析

    • Flow cytometric analysis of Human CD21 expression on Raji. Cells from the Raji (Human Burkitt's lymphoma B lymphocyte, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD21 Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

  • 实验方案