12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
WB result of Tau (phospho S396) Rabbit mAb
Primary antibody: Tau (phospho S396) Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse brain lysate 20 µg (treated with phosphatase)
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 79 kDa
Observed MW: 50 kDa
(This blot was developed with high sensitivity substrate)
Dot blot result of Tau (phospho S396) Rabbit mAb
Lane 1: Tau (phospho S396) peptide
Lane 2: Tau (phospho S262) peptide
Lane 3: Tau unmodified peptide
Primary antibody: Tau (phospho S396) Rabbit mAb at 1/500 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Exposure time: 180 s (This blot was developed with high sensitivity substrate)
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