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Alexa Fluor® 647 Mouse Anti-Human CD9 Antibody (S-632-33)

Alexa Fluor® 647 Mouse Anti-Human CD9 Antibody (S-632-33)

货号: S0B0173
价格: 650
规格: 20T
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    CD9
  • 分子别名

    CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29 (Tspan-29), p24, MIC3, TSPAN29
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cell membrane
  • Accession

    P21926
  • 克隆号

    S-632-33
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1,k
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein G
  • 浓度

    20μg/ml
  • 标记

    Alexa Fluor® 647
  • 性状

    Liquid
  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度
FCM 5 μl per million cells in 100μl volume
背景介绍
  • CD9 belongs to the cell surface glycoprotein cross -membrane four -protein family, with four cross -membrane domains, a shorter outer domain (ECL1), and a longer extracellular domain (ECL2). It is expressed in a variety of hematopoietic cells and epithelial cells. For example, Pre B cells, B cell subset, activated T cells, basophils, eosinophils, macrophages, megakaryocytes, plasma cells, plasma cell precursors in germinal centers, and platelets. Broadcasting a series of cell processes such as cell adhesion, movement, membrane tissue and signal transfusion. The lowering of CD9 expression is related to the adverse prognosis and progress of various cancers. It is a favorable marker of gallbladder cancer, gastic GIST, malignant cortex and oral squamous cell carcinoma.

  • 流式分析

    • Flow cytometric analysis of Human CD9 expression on HCT116. Cells from the HCT116 (Human colorectal carcinoma epithelial cell, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD9 Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
  • 实验方案