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Fer Recombinant Rabbit mAb (S-R303)

Fer Recombinant Rabbit mAb (S-R303)

货号: S0B0432
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Fer

  • 分子别名

    Tyrosine-protein kinase Fer, Feline encephalitis virus-related kinase FER, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Proto-oncogene c-Fer, Tyrosine kinase 3, p94-Fer, TYK3

  • 细胞定位

    Cell membrane, Cytoplasm, Nucleus

  • Accession

    P16591

  • 克隆号

    S-R303

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICFCM 1:50
ICC 1:500
背景介绍

  • Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.

  • 免疫印迹

    • WB result of Fer Rabbit mAb
      Primary antibody: Fer Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: A549 whole cell lysate 20 µg
      Lane 4: LNCaP whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 95 kDa
      Observed MW: 95 kDa
      (This blot was developed with high sensitivity substrate)

    • WB result of Fer Rabbit mAb
      Primary antibody: Fer Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 95 kDa
      Observed MW: 95 kDa

    • WB result of Fer Rabbit mAb                 

      Primary antibody: Fer Rabbit mAb at 1/1000 dilution

      Lane 1: NIH/3T3 whole cell lysate 5 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 95 kDa

      Observed MW: 95 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Fer antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Fer antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).