12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:2000 |
| IHC-P | 1:8000 |
Lactate dehydrogenase (LDH) is mainly responsible for catalyzing the conversion of pyruvate into lactate. It is present in almost all living cells. It consists of four subunits forming a tetramer and can form five different subtypes of LDH. Their enzymatic properties are similar, but there are obvious specific differences in tissue distribution. LDH-M and LDH-H subunits are encoded by the LDHA and LDHB genes and can also be expressed as LDHA and LDHB. LDHC is the testis-specific LDH protein.
WB result of LDH-C/A Rabbit mAb
Primary antibody: LDH-C/A Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDa
WB result of LDH-C/A Rabbit mAb
Primary antibody: LDH-C/A Rabbit mAb at 1/2000 dilution
Lane 1: mouse liver lysate 5 µg
Lane 2: mouse testis lysate 5 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDa
Exposure time: 30 s
IHC shows positive staining in paraffin-embedded human testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-LDH-C/A antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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