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eRF3/GSPT1 Recombinant Rabbit mAb (S-R325)

eRF3/GSPT1 Recombinant Rabbit mAb (S-R325)

货号: S0B0414
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    eRF3/GSPT1

  • 分子别名

    Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog

  • 细胞定位

    Cytoplasm

  • Accession

    P15170

  • 克隆号

    S-R325

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:500
IP 1:50
背景介绍

  • GSPT1 is a protein involved in the termination of translation, a process in which ribosomes synthesize proteins after the transcription of DNA to RNA. There is a demonstrated link between GSPT1 degradation and antitumor activity.

  • 免疫印迹

    • WB result of eRF3/GSPT1 Rabbit mAb
      Primary antibody: eRF3/GSPT1 Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Lane 3: PC-3 whole cell lysate 20 µg
      Lane 4: A431 whole cell lysate 20 µg
      Lane 5: HepG2 whole cell lysate 20 µg
      Lane 6: SK-OV-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 80 kDa

    • WB result of eRF3/GSPT1 Rabbit mAb
      Primary antibody: eRF3/GSPT1 Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: Raw 264.7 whole cell lysate 20 µg
      Lane 3: mouse brain lysate 20 µg
      Lane 4: mouse testis lysate 20 µg
      Lane 5: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 80 kDa

    • WB result of eRF3/GSPT1 Rabbit mAb
      Primary antibody: eRF3/GSPT1 Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Lane 2: rat testis lysate 20 µg
      Lane 3: rat spleen lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 55 kDa
      Observed MW: 80 kDa

  • 免疫沉淀

    • eRF3/GSPT1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating eRF3/GSPT1 in 0.4 mg PC-12 whole cell lysate.
      Western blot was performed on the immunoprecipitate using eRF3/GSPT1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: PC-12 whole cell lysate 20 µg (Input)
      Lane 2: eRF3/GSPT1 Rabbit mAb IP in PC-12 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in PC-12 whole cell lysate
      Predicted MW: 55 kDa
      Observed MW: 80 kDa

  • 免疫细胞化学

    • ICC shows positive staining in PC-3 cells. Anti-eRF3 GSPT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).