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Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody (S-R058)

Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody (S-R058)

货号: S0B0161
价格: 650
规格: 100μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 克隆号

    S-R058
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    FCM
  • 纯化方式

    Protein A
  • 浓度

    0.2 mg/ml
  • 标记

    Alexa Fluor® 488
  • 性状

    Liquid
  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}, {"application": "ChIP", "dilution": "", "species": ""}]
背景介绍
  • Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

  • 流式分析

    • Flow cytometric analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labeling SDT Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT CD38 antibody at 1 μg/test (S0B0092) (Ritht) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labeling SDT Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT β-tubulin antibody at 1 μg/test dilution (S0B0132) (Right) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.