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KRAS Recombinant Rabbit mAb (S-R291)

KRAS Recombinant Rabbit mAb (S-R291)

货号: S0B0403
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    KRAS
  • 分子别名

    GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, KRAS2, RASK2
  • 细胞定位

    Cytoplasm, Cell membrane
  • Accession

    P01116
  • 克隆号

    S-R291
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    ICFCM, ICC, WB, IP
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:2000
ICC 1:500
ICFCM 1:50
IP 1:50
背景介绍
  • The K-Ras protein is a GTPase, a class of enzymes which convert the nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). In this way the K-Ras protein acts like a switch that is turned on and off by the GTP and GDP molecules. To transmit signals, it must be turned on by attaching (binding) to a molecule of GTP. The K-Ras protein is turned off (inactivated) when it converts the GTP to GDP. When the protein is bound to GDP, it does not relay signals to the nucleus. Like other members of the ras subfamily of GTPases, the K-Ras protein is an early player in many signal transduction pathways. Once it is allosterically activated, it recruits and activates proteins necessary for the propagation of growth factors, as well as other cell signaling receptors like c-Raf and PI 3-kinase.

  • 免疫印迹

    • WB result of KRAS Rabbit mAb
      Primary antibody: KRAS Rabbit mAb at 1/2000 dilution
      Lane 1: SW480 whole cell lysate 20 µg
      Lane 2: Caco-2 whole cell lysate 20 µg
      Lane 3: HCT 116 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 21, 23 kDa

    • WB result of KRAS Rabbit mAb
      Primary antibody: KRAS Rabbit mAb at 1/2000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 21, 23 kDa

    • WB result of KRAS Rabbit mAb
      Primary antibody: KRAS Rabbit mAb at 1/2000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 22 kDa
      Observed MW: 21 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling KRAS antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • KRAS Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating KRAS in 0.4 mg HCT-116 whole cell lysate.
      Western blot was performed on the immunoprecipitate using KRAS Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HCT-116 whole cell lysate 20 µg (Input)
      Lane 2: KRAS Rabbit mAb IP in HCT-116 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HCT-116 whole cell lysate
      Predicted MW: 22 kDa
      Observed MW: 21, 23 kDa

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-KRAS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

    • ICC shows positive staining in NIH/3T3 cells. Anti-KRAS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).