12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC | 1:200-1:500 |
| IF | 1:200 |
| WB | 1:1000 |
| IP | 1:50 |
Homeobox protein Nkx-6.1 is a protein that in humans is encoded by the NKX6-1 gene. In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes.
WB result of NKX6.1 Rabbit mAb
Primary antibody: NKX6.1 Rabbit mAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Lane 2: MIN6 whole cell lysate 20 µg
Negative control: RAW 264.7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 44, 46 kDa
NKX6.1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating NKX6.1 in 0.4 mg β-TC-6 whole cell lysate.
Western blot was performed on the immunoprecipitate using NKX6.1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: β-TC-6 whole cell lysate 20 µg (Input)
Lane 2: NKX6.1 Rabbit mAb IP in β-TC-6 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in β-TC-6 whole cell lysate
Predicted MW: 38 kDa
Observed MW: 44,46 kDa
IHC shows positive staining in paraffin-embedded human pancreas. Anti-NKX6.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-NKX6.1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded human pancreas. Anti-NKX6.1 antibody was used at 1/200 dilution (green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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