12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IF | 1:200 |
| FCM | 1:250 |
CD8α encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Flow cytometric analysis of mouse primary splenocytes labeling CD8α (Alexa Fluor® 594 Conjugate) at 1/250 dilution (right panel) compared with a Rabbit IgG, monoclonal Isotype Control (left panel).
Then cells were stained with CD4 - APC/Cy7 separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
IF shows positive staining in paraffin-embedded mouse thymus. Anti-CD8α (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
您现在的位置：