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eIF3B Rabbit polyclonal antibody

eIF3B Rabbit polyclonal antibody

货号: S0B0384
价格: 480
规格: 25μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    eIF3B

  • 分子别名

    Eukaryotic translation initiation factor 3 subunit B, Eukaryotic translation initiation factor 3 subunit 9, Prt1 homolog (hPrt1), eIF-3-eta, eIF3 p110, eIF3 p116, EIF3S9

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P55884

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Bv

  • 纯化方式

    Immunogen Affinity

  • 浓度

    Lot specific* (generally 0.5 to 5 mg/ml)*

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IHC 1:200
背景介绍

  • Translation initiation factor 3 (eIF3), the largest, most complex translation initiation factor, is involved in almost the entire process of translation initiation regulation, including ribosomal recruitment of the ternary complex, attachment of 43S complexes to mRNA via interaction with eIF4G, selection of the initiation codon (AUG), and prevention of premature association of the 40 and 60 S ribosomal subunits.

  • 免疫印迹

    • WB result of eIF3B Rabbit polyclonal antibody
      Primary antibody: eIF3B Rabbit polyclonal antibody at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Lane 4: A431 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 92 kDa
      Observed MW: 120 kDa

    • WB result of eIF3B Rabbit polyclonal antibody
      Primary antibody: eIF3B Rabbit polyclonal antibody at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 92 kDa
      Observed MW: 120 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-eIF3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.