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β-Catenin Recombinant Rabbit mAb (Cy5 Conjugate) (S-336-23)

β-Catenin Recombinant Rabbit mAb (Cy5 Conjugate) (S-336-23)

货号: S0B0149
价格: 458
规格: 10μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    β-Catenin
  • 分子别名

    Catenin beta-1, Beta-catenin,CTNNB1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cell membrane, Cytoplasm
  • Accession

    P35222
  • 克隆号

    S-336-23
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 应用

    IF
  • 反应种属 ?

    Hu, Ms, Rt
  • 纯化方式

    Protein A
  • 浓度

    2 mg/ml
  • 标记

    Cy5
  • 性状

    Liquid
  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied

稀释度
应用 稀释度
IF 1:200
背景介绍
  • β-catenin is a multifunction protein with a central role in physiological homeostasis. Its abnormal expression leads to various diseases including cancer. In normal physiology, β-catenin either maintains integrity of epithelial tissues or controls transcription of various genes on extracellular instigations. In epithelial tissues, β-catenin functions as a component of the cadherin protein complex and regulates epithelial cell growth and intracellular adhesion [PMID: 23490077].

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human breast cancer. Anti-β-Catenin (Cy5 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded mouse liver. Anti-β-Catenin (Cy5 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat liver. Anti-β-Catenin (Cy5 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.