您现在的位置: 首页   -  产品中心   -  免疫学   -   抗体
FITC Mouse Anti-Mouse H-2Kb Antibody (S-R659)

FITC Mouse Anti-Mouse H-2Kb Antibody (S-R659)

货号: S0B5056
价格: 300
规格: 20T
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    Mouse H-2Kb
  • 分子别名

    H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K1; H2-K
  • 细胞定位

    Membrane
  • Accession

    P01901
  • 克隆号

    S-R659
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG2a,k
  • 应用

    FCM
  • 反应种属 ?

    Ms
  • 阳性样本

    C57BL/6 mouse splenocytes
  • 纯化方式

    Protein A
  • 浓度

    50 μg/ml
  • 标记

    FITC
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
稀释度
应用 稀释度 推荐种属
FCM 5μl per million cells in 100μl volume Ms
背景介绍
  • H-2Kb protein is a major histocompatibility class I molecule expressed in mice, playing a crucial role in antigen presentation and immune response. It is involved in the processing and presentation of endogenous and exogenous peptide antigens via the MHC class I pathway, facilitating T cell-mediated cytotoxicity. H-2Kb is also implicated in the regulation of local cortical circuits in the brain, with its absence leading to excess connectivity that can function as a substrate for cortical plasticity. Additionally, H-2Kb has been studied in the context of viral infections, such as vesicular stomatitis virus, where it forms complexes with viral peptides and beta-2 microglobulin, providing insights into the structural basis of antigen recognition.

  • 流式分析

    • Flow cytometric analysis of Mouse H-2Kb expression on C57BL/6 mouse splenocytes. Cells from the C57BL/6 mouse splenocytes (Right) or BALB/c mouse splenocytes (Left) were stained with either FITC Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT FITC Mouse Anti-Mouse H-2Kb Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.