PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Ms |
H-2Kd protein is a major histocompatibility complex (MHC) class I molecule expressed in mice, particularly in BALB/c strains. It plays a critical role in antigen presentation by binding to antigenic peptides derived from pathogens, such as influenza nucleoprotein, and presenting them to CD8+ T cells to initiate an immune response. The crystal structure of H-2Kd in complex with beta-2 microglobulin and an influenza-derived peptide has been elucidated, revealing specific binding motifs and the structural basis for peptide recognition. This understanding is crucial for studies on immune responses and vaccine development.
Flow cytometric analysis of Mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with FITC Mouse IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Mouse H-2Kd Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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