PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu |
CD68 is a 110-kD transmembrane glycoprotein highly expressed by human monocytes, macrophages, and cells of the monocyte-macrophage lineage, such as osteoclasts and microglia. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and the scavenger receptor family. CD68 primarily localizes to lysosomes and endosomes, with a smaller fraction circulating to the cell surface. It plays a role in promoting phagocytosis and clearing cellular debris. The protein is also involved in mediating the recruitment and activation of macrophages.
Human PBMC (human peripheral blood mononuclear cells) were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Alexa Fluor® 647 Mouse Anti-Human CD68 Antibody at 5μl/test / (Right panel) compared with a Mouse IgG2b, κ Isotype Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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