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M13 OneStep ELISA Kit

M13 OneStep ELISA Kit

货号: S0C3033
价格: 3200
规格: 1x96T
介绍: -
其他: -
产品规格
  • 抗原名称

    M13 Phage
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Others
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.
  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      板内变异系数 (Intra-assay): 6.1%;
      板间变异系数 (Inter-assay): 7.5%
    • Sample type

      Cell culture supernatant
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      7.079E+03 particles /ml
    • Range

      2.205E+04 particles /ml – 2.500E+06 particles /ml
    • Recovery

      Cell culture supernatant: 92%
    • Assay time

      60 minutes
背景介绍
  • M13 is a filamentous phage that has attracted much attention in the field of molecular biology and biotechnology. It belongs to the family Inoviridae and mainly infects Escherichia coli (E. coli). M13 phage has a simple single-stranded DNA genome of about 6.4 kilobases and a rod-shaped morphology. M13 phage can display foreign peptides or proteins on its surface. By genetically engineering the M13 genome to include genes encoding proteins of interest in the phage coat protein genes, phage particles are generated that can display proteins on their surface. Phage titration ELISA is a technique used to determine the concentration or titer of phage (bacteriophage) particles in a given sample. The Phage Titration ELISA determines the relative number of phage particles and provides a rapid, sensitive and reproducible alternative method for titrating M13 phage preparations.

  • ELISA

    • Standard curve
      Example of M13 standard curve in Assay Diluent #1.

    • Leading Competitor comparison

    • Protocol Diagram