PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu |
CD278, also known as ICOS (inducible T-cell co-stimulator), is a member of the CD28 superfamily and is expressed on activated T cells, including regulatory T cells (Tregs) and follicular helper T cells (Tfh). It functions as a homodimer and plays a crucial role in T cell activation, proliferation, and differentiation. ICOS binds to its ligand ICOSL (CD275), which is expressed on antigen-presenting cells (APCs) such as dendritic cells, B cells, and macrophages. This interaction enhances T cell-mediated immune responses, promotes cytokine production (e.g., IL-4, IL-13), and supports the survival and function of Tfh cells, which are critical for B cell antibody production. ICOS signaling is also involved in Th2 and Th17 cell differentiation and has been implicated in autoimmune and inflammatory diseases. In cancer immunotherapy, ICOS agonist antibodies have shown potential for enhancing anti-tumor immune responses when combined with other immunotherapies.
Treated 48h with 10μg/ml PHA human PBMC (human peripheral blood mononuclear cell) (Right panel) or untreated (Left panel) was stained with Alexa Fluor® 647 Mouse Anti-Human CD3 and SDT FITC Armenian Hamster Anti-Human CD278 Antibody at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Treated overnight with 3μg/ml Concanavalin A C57BL/6 mouse splenocytes (Right panel) or untreated (Left panel) was stained with Alexa Fluor® 647 Rat Anti-Mouse CD3 and SDT FITC Armenian Hamster Anti-Human CD278 Antibody at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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