PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICFCM | 5μl per million cells in 100μl volume | Hu, Ms |
Helios protein (encoded by the IKZF2 gene) is a member of the Ikaros family of transcription factors and contains multiple zinc finger domains that facilitate DNA binding and the formation of homodimers or heterodimers. It is predominantly expressed in T cells, regulatory T cells (Tregs), certain B cell subsets, and hematopoietic and epithelial tissues. Helios plays a crucial role in T cell activation, proliferation, and the stability and function of Tregs. Additionally, Helios has been identified as a potential biomarker in autoimmune diseases and may influence tumor development by regulating gene expression and modulating cell differentiation and proliferation.
Human PBMC (human peripheral blood mononuclear cells) were stained with Alexa Fluor® 647 Mouse Anti-Human CD4 antibody and fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent. The cells were then stained with either FITC Armenian hamster IgG Isotype Control (Left panel) or SDT FITC Armenian hamster Anti-Mouse/Human Helios Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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