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FITC Mouse Anti-Human CD14 Antibody (S-631-50)

FITC Mouse Anti-Human CD14 Antibody (S-631-50)

货号: S0B5028
价格: 402
规格: 25T
介绍: -
其他: -
产品规格
  • 宿主来源

    Mouse
  • 抗原名称

    CD14
  • 分子别名

    Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Secreted, Cell membrane
  • Accession

    P08571
  • 克隆号

    S-631-50
  • 抗体类型

    Mouse mAb
  • 抗体同种型

    IgG1,k
  • 应用

    FCM
  • 反应种属 ?

    Hu
  • 阳性样本

    human PBMC
  • 纯化方式

    Protein G
  • 浓度

    0.2mg/ml
  • 标记

    FITC
  • 性状

    Liquid
  • 缓冲体系

    PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度
应用 稀释度 推荐种属
FCM 1.25μlμl per million cells in 100μl volume Hu
背景介绍
  • CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol (GPI) tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).

  • 流式分析

    • Flow cytometric analysis of Human CD14 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD14 Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.