PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Caspase-9 is a key initiator caspase in the intrinsic apoptotic pathway, activated by the formation of the apoptosome complex in response to cellular stress such as DNA damage or mitochondrial dysfunction. It is synthesized as an inactive zymogen and becomes active upon recruitment by the apoptosome, which includes cytochrome c and apoptotic protease activating factor-1 (Apaf-1). Once activated, Caspase-9 cleaves and activates downstream effector caspases like Caspase-3, -6, and -7, ultimately leading to apoptosis. Caspase-9 is essential for maintaining cellular homeostasis and preventing the development of diseases such as cancer. Its dysregulation has been implicated in conditions like neurodegenerative disorders and tumorigenesis, making it a potential target for therapeutic interventions.
WB result of Caspase-9 Recombinant Rabbit mAb
Primary antibody: Caspase-9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 1 µM Staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 46 kDa
Observed MW: 40, 37, 30 kDa
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