PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Ms |
DLL4 (Delta-like ligand 4) is a key Notch ligand involved in the regulation of vascular development and angiogenesis. It is predominantly expressed in endothelial cells and plays a crucial role in the formation and maintenance of arterial-venous identity during embryonic development. DLL4-mediated Notch signaling is essential for proper vascular morphogenesis, as evidenced by severe vascular defects in DLL4-deficient embryos. In addition to its developmental roles, DLL4 is implicated in various pathological conditions, including cancer. High expression of DLL4 has been observed in certain cancers, such as testicular and renal cancer, where it may contribute to tumor angiogenesis and progression. DLL4 also modulates macrophage function through the Notch signaling pathway, influencing immune responses and tissue repair. Recent studies have also highlighted the role of DLL4 in skeletal muscle, where it regulates muscle mass through interactions with Notch receptors in muscle cells. Overall, DLL4 is a critical regulator of Notch signaling in both physiological and pathological contexts, making it a potential target for therapeutic interventions.
WB result of DLL4 Recombinant Rabbit mAb
Primary antibody: DLL4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 80 kDa
WB result of DLL4 Recombinant Rabbit mAb
Primary antibody: DLL4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 80 kDa
WB result of DLL4 Recombinant Rabbit mAb
Primary antibody: DLL4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 80 kDa
IF shows positive staining in paraffin-embedded mouse lung. Anti-DLL4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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