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Lactate Dehydrogenase Rabbit Polyclonal Antibody

Lactate Dehydrogenase Rabbit Polyclonal Antibody

货号: S0B1351
价格: 600
规格: 50μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Lactate Dehydrogenase
  • 分子别名

    L-lactate dehydrogenase A chain; LDH-A; Cell proliferation-inducing gene 19 protein; LDH muscle subunit (LDH-M); Renal carcinoma antigen NY-REN-59; LDHA; PIG19; L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit (LDH-H); Renal carcinoma antigen NY-REN-46; LDHB
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm
  • Accession

    P00338, P07195
  • 抗体类型

    Polyclonal antibody
  • 抗体同种型

    IgG
  • 应用

    IHC-P, ICC, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    KATO III, HeLa, HCC827, NIH/3T3, mouse heart, mouse skeletal muscle, C6, rat heart, rat skeletal muscle
  • 预测反应种属
    (反应种属缩写表)

    Cz,Bv
  • 纯化方式

    Immunogen Affinity
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Ms, Rt
ICC 1:500 Hu
背景介绍
  • Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate, playing a crucial role in anaerobic glycolysis and energy metabolism. It exists as five isoenzymes (LDH-1 to LDH-5), which are tetramers composed of different combinations of M and H subunits. LDH is widely expressed in various tissues, with different isoenzymes having distinct tissue distributions and functions. For instance, LDH-1 is abundant in the heart, while LDH-5 is prevalent in skeletal muscle and liver. In addition to its metabolic role, LDH is a biomarker for tissue damage, as its release into the bloodstream indicates cell injury or necrosis. Elevated LDH levels are commonly observed in conditions such as myocardial infarction, liver disease, and hemolysis. In cancer, LDH is involved in the Warburg effect, promoting tumor growth and survival by facilitating lactate production under hypoxic conditions. LDH also contributes to the acidic tumor microenvironment, which supports immune evasion and metastasis.

  • 免疫印迹

    • WB result of Lactate Dehydrogenase Rabbit pAb
      Primary antibody: Lactate Dehydrogenase Rabbit pAb at 1/1000 dilution
      Lane 1: KATO Ⅲ whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HCC827 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 35 kDa

    • WB result of Lactate Dehydrogenase Rabbit pAb
      Primary antibody: Lactate Dehydrogenase Rabbit pAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse heart lysate 20 µg
      Lane 3: mouse skeletal muscle lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 35 kDa

    • WB result of Lactate Dehydrogenase Rabbit pAb
      Primary antibody: Lactate Dehydrogenase Rabbit pAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat heart lysate 20 µg
      Lane 3: rat skeletal muscle lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 37 kDa
      Observed MW: 35 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Lactate Dehydrogenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Lactate Dehydrogenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-Lactate Dehydrogenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Lactate Dehydrogenase antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Lactate Dehydrogenase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Lactate Dehydrogenase in mouse & rat tissue.