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RNF40 Recombinant Rabbit mAb (S-1068-279)

RNF40 Recombinant Rabbit mAb (S-1068-279)

货号: S0B1356
价格: 600
规格: 50μl
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    RNF40

  • 分子别名

    E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein (RBP95); RING finger protein 40; RING-type E3 ubiquitin transferase BRE1B; BRE1B; KIAA0661

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Nucleus

  • Accession

    O75150

  • 克隆号

    S-1068-279

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    MCF7, HeLa, HCT 116, HepG2, RAW264.7, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
背景介绍

  • RNF40 (Ring Finger Protein 40) is an E3 ubiquitin ligase that plays a critical role in histone H2B monoubiquitination at lysine 120 (H2Bub1), which is essential for gene regulation and chromatin remodeling. This protein is involved in various biological processes, including DNA damage repair, apoptosis, and cancer development. Recent studies have highlighted its dual roles in cancer progression. For example, in triple-negative breast cancer (TNBC), RNF40 supports cancer stem cell properties and glycolytic programs, promoting tumor aggressiveness. Additionally, RNF40 regulates lipid metabolism by targeting LIMA1 for proteasome-mediated degradation, thereby influencing cellular sensitivity to lipid synthesis inhibitors. These findings underscore RNF40's multifaceted roles in epigenetic regulation and cancer biology, making it a potential therapeutic target.

  • 免疫印迹

    • WB result of RNF40 Recombinant Rabbit mAb
      Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HCT 116 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 130 kDa

    • WB result of RNF40 Recombinant Rabbit mAb
      Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 130 kDa

    • WB result of RNF40 Recombinant Rabbit mAb
      Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 130 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of RNF40 in tumor tissue.

    • Expression of RNF40 in human tissue.

    • Expression of RNF40 in mouse & rat tissue.