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CDK9 Rabbit Polyclonal Antibody

CDK9 Rabbit Polyclonal Antibody

货号: S0B1331
价格: 400
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    CDK9
  • 分子别名

    Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; CDC2L4; TAK
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Nucleus
  • Accession

    P50750
  • 抗体类型

    Polyclonal antibody
  • 抗体同种型

    IgG
  • 应用

    IHC-P, WB
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HT-29, HeLa, HL-60, 293T, NIH/3T3, RAW264.7, mouse, mouse liver, PC-12, C6, rat testis
  • 预测反应种属
    (反应种属缩写表)

    Bv
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
背景介绍
  • CDK9 protein, located at 9q34.11 and consisting of 372 amino acids, is a CDC2-related kinase and the catalytic subunit of the positive-transcription elongation factor b (P-TEFb). It was first identified in 1994 and is primarily involved in regulating the elongation phase of transcription by RNA polymerase II (RNAPII). CDK9 forms complexes with cyclin T1, T2a, T2b, or K, and these complexes phosphorylate the C-terminal domain (CTD) of RNAPII and negative-transcription elongation factors, thereby overcoming promoter-proximal pausing and facilitating transcription elongation. CDK9 is also involved in transcription initiation and termination, and its activity is regulated by post-translational modifications such as phosphorylation and acetylation. Additionally, CDK9 has been implicated in various cellular processes, including immune responses and metabolic regulation, and is a potential therapeutic target in cancer and other diseases.

  • 免疫印迹

    • WB result of CDK9 Rabbit pAb
      Primary antibody: CDK9 Rabbit pAb at 1/1000 dilution
      Lane 1: HT-29 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HL-60 whole cell lysate 20 µg
      Lane 4: 293T whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 43, 53 kDa
      Observed MW: 38, 50 kDa

    • WB result of CDK9 Rabbit pAb
      Primary antibody: CDK9 Rabbit pAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: RAW264.7 whole cell lysate 20 µg
      Lane 3: mouse testis lysate 20 µg
      Lane 4: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 43, 53 kDa
      Observed MW: 38, 50 kDa

    • WB result of CDK9 Rabbit pAb
      Primary antibody: CDK9 Rabbit pAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: C6 whole cell lysate 20 µg
      Lane 3: rat testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 43, 53 kDa
      Observed MW: 38, 50 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.