PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Species Independent |
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
WB result of His tag (C-terminal) Mouse mAb
Primary antibody: His tag (C-terminal) Mouse mAb at 1/1000 dilution
Lane 1: 4-1BB/TNFRSF9 His Tag Protein, Human 10 ng
Lane 2: Angiopoietin-1 (277-498) His Tag Protein, Mouse 10 ng
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28~33 kDa
Observed MW: 30 kDa
WB result of His tag (C-terminal) Mouse mAb
Primary antibody: His tag (C-terminal) Mouse mAb at 1/1000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with Histone H3-myc-His tag fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 20 kDa
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