PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu |
CD59, also known as protectin, is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of various cells. It plays a vital role in regulating the immune system and protecting cells from the complement system. Specifically, CD59 is the only membrane-bound inhibitor of the complement membrane attack complex (MAC), an immune pore that can damage human cells. Without CD59, MAC pores formed on red blood cells result in rupture, causing chronic hemolysis and immune-related neuropathies.
Flow cytometric analysis of Human CD59 expression on human peripheral blood. Human peripheral blood was stained with Alexa Fluor® 647 Mouse IgG2a Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD59 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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