PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000-1:10000 | Species Independent |
Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose. Once the fusion protein is obtained in purified form, the protein of interest is often cleaved from MBP with a specific protease and can then be separated from MBP by affinity chromatography.
WB result of MBP tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: 293T transfected with Histone H3-MBP tag fusion protein whole cell lysate 20 µg
Primary antibody:
Lane 1: MBP tag Mouse mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: MBP tag Mouse mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: MBP tag Mouse mAb (HRP Conjugate) at 1/50000 dilution
Predicted MW: 43 kDa
Observed MW: 60 kDa
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