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Rabbit Anti-His Tag (C-terminal) Magnetic Agarose

Rabbit Anti-His Tag (C-terminal) Magnetic Agarose

货号: S0B1827
价格: 800
规格: 125μl(5reactions)
介绍: -
其他: -
产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    His tag

  • 免疫原

    Synthetic Peptide

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    Co-IP, Protein purification, IP

  • 反应种属 ?

    Species Independent

  • 纯化方式

    Protein A

  • 标记

    Magnetic Agarose

  • 性状

    Liquid

  • 缓冲体系

    20% slurry in PBS pH7.4, 0.05% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
背景介绍

  • Rabbit Anti-His Tag (C-terminal) Magnetic Agarose is an affinity chromatography medium designed for easy, one-step purification of recombinant His tag (C-terminal) fusion proteins in E. coli, insect cells and mammalian cells. The recombinant His tag (C-terminal) fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-His Tag (C-terminal) Magnetic Agarose. It is the excellent choice for high performance purifications.

  • 电泳JSON

    • Rabbit Anti-His Tag (C-terminal) Magnetic Agarose is used to immunoprecipitate His-tagged fusion proteins from HEK293T cell lysates. During elution with Acidic elution buffer, the enriched His-tagged fusion proteins are released from the magnetic agarose.
      SDS-PAGE analysis was performed.
      Lane 1: Input
      Lane 2: Flow-through
      Lane 3: His-tagged fusion protein
      Lane 4: Rabbit IgG Isotype Control Magnetic Agarose

    • Rabbit Anti-His Tag (C-terminal) Magnetic Agarose is used to immunoprecipitate His-tagged fusion proteins from HEK293T cell lysates. During elution with 2x SDS-sample buffer, the enriched His-tagged fusion proteins and antibodies are released from the magnetic agarose.
      SDS-PAGE analysis was performed.
      Lane 1: Input
      Lane 2: Flow-through
      Lane 3: His-tagged fusion protein