PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Cleaved Caspase-9 (Asp330) is an activated form of Caspase-9 that results from proteolytic cleavage at the Asp330 site. This cleavage is a critical step in the intrinsic apoptosis pathway, where Caspase-9 is activated by the binding of cytochrome c to Apaf-1, leading to the formation of the apoptosome complex. The cleaved Caspase-9 then further activates downstream caspases such as Caspase-3 and Caspase-7, initiating a cascade that ultimately leads to apoptosis. This process is essential for the execution of programmed cell death in response to various cellular stresses and DNA damage.
WB result of Cleaved Caspase-9 (Asp330) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-9 (Asp330) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
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