PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Histone deacetylase 3 (HDAC3) is a class I HDAC that plays a critical role in chromatin remodeling and gene transcription regulation. It is unique among HDACs due to its ability to shuttle between the nucleus and cytoplasm, allowing it to exert both enzymatic and non-enzymatic functions. HDAC3 primarily functions as a co-repressor, interacting with nuclear receptor co-repressors (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRT) to deacetylate histones and repress gene expression. This activity is crucial for various physiological processes, including embryonic development, circadian rhythms, and metabolism. Additionally, HDAC3 is implicated in several diseases, such as cancer, neurodegeneration, and cardiovascular diseases, making it a target for therapeutic interventions. Its role in inflammation is also significant, as it regulates pro-inflammatory gene expression in macrophages. HDAC3 inhibitors are being explored for their potential in treating these conditions, with specific inhibitors showing promise in reducing side effects compared to general HDAC inhibitors.
WB result of HDAC3 Rabbit pAb
Primary antibody: HDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 48 kDa
WB result of HDAC3 Rabbit pAb
Primary antibody: HDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 48 kDa
WB result of HDAC3 Rabbit pAb
Primary antibody: HDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 48 kDa
ICC shows positive staining in NIH/3T3 cells. Anti- HDAC3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in K562 cells. Anti- HDAC3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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