PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Matrix metalloproteinase-7 (MMP-7) is a small proteolytic enzyme that is secreted as a zinc- and calcium-dependent endopeptidase. It plays a crucial role in the degradation of various extracellular matrix substrates and non-ECM proteins, such as E-cadherin and Fas ligand, which are involved in cell adhesion and apoptosis. MMP-7 is implicated in several physiological processes, including wound healing, bone growth, and remodeling. In cancer, MMP-7 is often overexpressed and contributes to tumor progression by promoting cell proliferation, invasion, metastasis, and angiogenesis. Its expression levels correlate with the clinical characteristics of cancer patients, making it a potential biomarker and therapeutic target for various types of cancer.
WB result of MMP7 Recombinant Rabbit mAb
Primary antibody: MMP7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 25 kDa
WB result of MMP7 Recombinant Rabbit mAb
Primary antibody: MMP7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 23, 25 kDa
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human liver. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of MMP7 in tumor tissue.
Expression of MMP7 in human tissue.
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