PBS pH7.4
12 months from date of receipt / reconstitution, 2 to 8°C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Ms |
| FCM | 1:150 | Ms |
CD44 is a transmembrane glycoprotein that plays a crucial role in cell-cell and cell-matrix interactions. It is widely expressed on various cell types, including endothelial cells, epithelial cells, fibroblasts, and leukocytes. The molecule is composed of an extracellular domain, a transmembrane domain, and an intracellular domain, with the extracellular domain being responsible for binding to ligands such as hyaluronic acid (HA), which is its primary ligand. CD44 is involved in numerous physiological processes, including cell adhesion, migration, proliferation, and immune cell activation. Additionally, it plays a significant role in cancer progression, as it is often overexpressed in cancer cells and contributes to tumor growth, invasion, and metastasis. The diverse functions of CD44 are attributed to its various isoforms, which result from alternative splicing and post-translational modifications.
Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / C2C12 (Mouse myoblasts myoblast, Right) labelling SDT Rat Anti-Mouse CD44 Antibody at 1/150 dilution (1 μg) / (Red) compared with a Rat IgG (Black) Isotype control. Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: Neuro-2a
ICC shows positive staining in C2C12 cells (top panel) and negative staining in Neuro-2a cells (below panel). Anti-CD44 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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