PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
RBM15B, also known as RNA binding motif protein 15B, is a member of the SPEN (Split-end) family of proteins and is involved in various cellular processes. It functions as an RNA-binding protein that plays a key role in regulating N6-methyladenosine (m6A) methylation of RNAs, which affects mRNA splicing and processing. RBM15B is also implicated in the regulation of alternative splicing and acts as a cofactor for the nuclear export receptor NXF1, influencing mRNA export from the nucleus. Additionally, it has been associated with diseases such as Tatton-Brown-Rahman Syndrome and Wilms Tumor 1, highlighting its importance in both normal and pathological cellular functions.
WB result of RBM15B Recombinant Rabbit mAb
Primary antibody: RBM15B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 97 kDa
Observed MW: 100 kDa
IHC shows positive staining in paraffin-embedded human stomach. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of RBM15B in tumor tissue.
Expression of RBM15B in human tissue.
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