PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
SMARCD1, also known as BAF60A, is a protein that is part of the SWI/SNF family of chromatin remodeling complexes. It plays a crucial role in regulating gene transcription by altering chromatin structure, which involves the alteration of DNA-nucleosome topology in an ATP-dependent manner. SMARCD1 is involved in various biological processes, including neurogenesis and the regulation of lipid metabolism. It is also implicated in diseases such as Coffin-Siris Syndrome and has been identified as a prognostic marker in liver hepatocellular carcinoma. The protein is ubiquitously expressed in the nucleus of various tissues and is essential for the activation of specific gene signatures in multipotent progenitors.
WB result of SMARCD1 Rabbit pAb
Primary antibody: SMARCD1 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
WB result of SMARCD1 Rabbit pAb
Primary antibody: SMARCD1 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
IHC shows positive staining in paraffin-embedded human testis. Anti-SMARCD1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-SMARCD1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-SMARCD1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in A431 cells. Anti- SMARCD1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SMARCD1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Expression of SMARCD1 in tumor tissue.
Expression of SMARCD1 in human tissue.
Expression of SMARCD1 in mouse & rat tissue.
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