PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:2000 | Hu |
| ICFCM | 1:800 | Hu |
Lamin A/C is a crucial component of the nuclear lamina, a fibrous network that lines the inner nuclear membrane. Encoded by the LMNA gene, lamin A/C is an intermediate filament protein that plays a significant role in maintaining the structural integrity and mechanical stability of the nucleus. Beyond its structural function, lamin A/C is involved in a variety of cellular processes, including DNA replication, gene transcription regulation, and cell differentiation. It interacts with numerous proteins and genomic regions, influencing gene expression by modulating the accessibility of DNA to transcription factors. Mutations in the LMNA gene can lead to a group of diseases known as laminopathies, which include muscular dystrophy, dilated cardiomyopathy, and progeria, a premature aging syndrome. These mutations often disrupt the normal function of lamin A/C, causing cellular dysfunction and tissue-specific pathologies.
Flow cytometric analysis of Lamin A/C expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with Alexa Fluor® 647 Rabbit IgG Isotype Control (Black line histogram) and SDT Lamin A/C Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
ICC shows positive staining in HeLa cells. Anti- Lamin A/C (Alexa Fluor® 647 Conjugate) antibody was used at 1/2000 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Green).
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