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Rat Anti-Mouse CD14 Antibody (S-R609)

Rat Anti-Mouse CD14 Antibody (S-R609)

货号: S0B1286
价格: 600
规格: 25μl
介绍: -
其他: -
产品规格
  • 宿主来源

    Rat
  • 抗原名称

    Mouse CD14
  • 分子别名

    Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
  • 细胞定位

    Cell membrane
  • Accession

    P10810
  • 克隆号

    S-R609
  • 抗体类型

    Rat mAb
  • 抗体同种型

    IgG2a,k
  • 应用

    FCM
  • 反应种属 ?

    Ms
  • 阳性样本

    RAW 264.7
  • 纯化方式

    Protein G
  • 浓度

    2 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS pH7.4
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度
应用 稀释度 推荐种属
FCM 1:200 Ms
背景介绍
  • CD14, also known as the LPS receptor, is a surface antigen belonging to the family of cell surface glycoproteins. It exists in two forms: membrane-bound CD14 (mCD14), which is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) tail, and soluble CD14 (sCD14), found in the plasma. CD14 is primarily expressed on monocytes, macrophages, and dendritic cells, and plays a critical role in the immune system by recognizing and binding to lipopolysaccharide (LPS) from Gram-negative bacteria, leading to cellular activation and the initiation of inflammatory responses.

  • 流式分析

    • Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) labelling Mouse CD14 antibody at 1/200 dilution (1 μg) / (Red) compared with a Rat IgG2a, κ isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
      Negative control: Neuro-2a