PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000-1:10000 | Ms,Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| IF | 1:500 | Hu, Ms |
SNAP25, or synaptosomal-associated protein 25, is a crucial component of the SNARE complex that plays a pivotal role in the molecular regulation of neurotransmitter release. It is primarily associated with the presynaptic membrane and is involved in vesicle docking and membrane fusion, which are essential for the release of neurotransmitters into the synaptic cleft. Recent research has also suggested a potential postsynaptic role for SNAP25, indicating its presence in the postsynaptic density (PSD), postsynaptic lateral membrane, and small vesicles in the postsynaptic cytoplasm, which may be implicated in synaptic plasticity. Furthermore, SNAP25 has been linked to the modulation of the gating characteristics of certain ion channels, such as the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. Its expression and structural abnormalities have been reported in various neurological disorders, emphasizing its importance in synaptic function and neurological health.
WB result of SNAP25 Recombinant Rabbit mAb
Primary antibody: SNAP25 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: mouse hippocampus lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDa
WB result of SNAP25 Recombinant Rabbit mAb
Primary antibody: SNAP25 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: rat hippocampus lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDa
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Expression of SNAP25 in tumor tissue.
Expression of SNAP25 in human tissue.
Expression of SNAP25 in mouse & rat tissue.
您现在的位置：