12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Transferrin, also known as TRF or Tf, is a major glycoprotein in the plasma of vertebrates that plays a critical role in the transport and metabolism of iron ions. It is synthesized mainly in the liver and is responsible for carrying iron absorbed from the digestive tract and iron released from the degradation of red blood cells. Transferrin enters the bone marrow in the form of TRF-Fe3+ complex, supplying iron for the production of mature red blood cells. This protein can carry two trivalent iron ions (Fe3+) per molecule and interacts with Fe3+ depending on pH; it binds Fe3+ efficiently at pH 7.4 and releases it under acidic conditions. Transferrin also has several physiological and biochemical functions, including antimicrobial activity, involvement in immune system cell protection, and regulation of cell proliferation. Moreover, it serves as a novel active targeting carrier in the field of tumor targeting, showing promise for future applications.
WB result of Transferrin Recombinant Rabbit mAb
Primary antibody: Transferrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: human plasma lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 80 kDa
IHC shows positive staining in paraffin-embedded human kidney. Anti-Transferrin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Transferrin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Transferrin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of Transferrin in tumor tissue.
Expression of Transferrin in human tissue.
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