PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 5 μl per million cells in 100μl volume | Ms |
The TCR β (T-cell receptor beta chain) is a crucial component of the T-cell receptor, which, together with the TCR α chain, forms the αβTCR on the surface of T cells. This receptor is responsible for recognizing and binding to antigenic peptide complexes presented by MHC molecules. The diversity of the TCR β chain is generated through a combination of random recombination of multiple germline gene segments, gene rearrangement, junctional diversity, N-region nucleotide insertions, and random pairing of α and β chains. These mechanisms collectively provide the TCR repertoire with immense diversity, enabling T cells to recognize a wide range of antigens.
Flow cytometric analysis of TCR β expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with SDT FITC Armenian Hamster Anti-Mouse TCR β Antibody at 0.25 μg/test and either Alexa Fluor® 647 Rat Anti-Mouse CD8a Antibody (Left panel) or Brilliant Violet 421™ Rat Anti-Mouse CD4 Antibody (Right panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.